EGFR Mutation
 
 

EGFR Mutation

Test name:
EGFR mutation

Order name:
EGFR

Specimen requirements:
Formalin-fixed paraffin embedded tissue (FFPE)

Minimum volume:
5-10 10µm sections of FFPE tissue in a sterile cup with stained H&E of same block.
If tumor percentage is less than 10% of total nuclei, macrodissection is required, send 5-10 10µm thickness unstained slides with stained H&E of same block.

Storage and Stability information:
Room temperature

Test performed:
Once per week

Methodology:
Real-time Polymerase Chain Reaction (PCR)

Reference range:
No EGFR mutation detected

Clinical significance:
Somatic mutations in the epidermal growth factor receptor (EGFR) gene have been associated with non-small cell lung cancer (NSCLC) patients’ responsiveness to tyrosine kinase inhibitors (TKI) (gefinitib, erlotinib). However, while some mutations have a sensitizing effect, other mutations are linked to TKI resistance. This test detects the following mutations: T790M, exon 19 deletions, L858R, G719X, exon 20 insertions, S768I, and L861Q.

CPT codes:
81401