Flow Cytometry
 
 

Flow Cytometry

Leukemia/Lymphoma Immunophenotyping

Immunophenotyping can help diagnose and classify blood cell cancers (leukemias and lymphomas). It may be ordered as a follow-up test when a white cell count differential shows an increased number of lymphocytes, the presence of immature white cells, an idiopathic change in the red cell indices or when there is a significant increase or decrease in the number of platelets (thrombocytosis or thrombocytopenia). Testing is most often performed on blood and/or bone marrow samples, but may also be done on body fluids or other biopsy tissue samples.

Immunophenotyping by Flow Cytometry is useful to:

  • Evaluate lymphocytosis of unknown etiology
  • Identify B- and T-cell lymphoproliferative disorders
  • Distinguish acute lymphoblastic leukemia (ALL) from acute myeloid leukemia (AML)
  • Subtype ALL
  • Distinguish reactive lymphocytes  from malignant lymphoma
  • Distinguish between malignant lymphoma and acute leukemia
  • Phenotype subclassification of B- and T-cell chronic lymphoproliferative disorders
  • Recognize AML with minimal morphologic evidence of differentiation

Lymphocyte Enumeration

The BayCare lymphocyte enumeration panel contains:

CD3 for the identification of T lymphocytes CD19 for the identification of B lymphocytes
CD16 for the identification of NK lymphocytes CD4 for the identification of T helper lymphocytes
CD56 for the identification of NK lymphocytes CD8 for the identification of T suppressor lymphocytes

An abbreviated CD4/CD8 panel is also available.

DNA Ploidy and S-Phase Cell Cycle Analysis

DNA ploidy and cell cycle analysis is a rapid and efficient way to evaluate the DNA content (ploidy) and proliferative activity (cell cycle/S-phase fraction) of cells. By staining the DNA with a fluorescent dye, flow cytometry can measure the dye fluorescence in many individual cells, and the data can be analyzed for ploidy (diploid/normal content or aneuploid/abnormal content) and proliferative activity.

Transfusion Related Cell Count

For more information, please call (800) 324-7853.