MYC/IGH t(8;14) by FISH

Test name:
FISH, MYC/IGH t(8;14)

Order name:
t(8;14) by FISH request

Specimen requirements:
Sodium heparin peripheral blood or bone marrow. Formalin fixed paraffin-embedded tissue (FFPE)

Minimum volume:
1mL blood or bone marrow. FFPE – 2 unstained 4µm thick sections on positively charged slides plus 1 stained H&E slide of the same block

Storage and stability information:
Room temperature 3 days for blood and bone marrow. FFPE at room temperature indefinitely.

Test performed:
Twice per week

Methodology:
Fluorescence in situ hybridization

Reference range:
See lab report

Clinical significance:
The t(8;14) translocation leads to juxtaposition of the Immunoglobulin Heavy Chain loci with the MYC gene.   The t(8;14) is associated with B-cell neoplasms, particularly Burkitt lymphoma/leukemia, although it is not specific for this neoplasm.  In addition, as the MYC gene can rearrange with other loci, the absence of the t(8;14) does not exclude the diagnosis of Burkitt lymphoma. 

CPT codes:
88368 x 2